NAD+

What is NAD+?

Nicotinamide Adenine Dinucleotide (NAD+) is a pyridine nucleotide, not a peptide, but is included in the research catalog because of its central role in cellular metabolism, sirtuin enzymology, and aging biology. NAD+ levels decline with age in many mammalian tissues, motivating substantial research into NAD+ precursors and bioavailability. Each batch is HPLC-verified for purity and form (oxidized vs reduced).

Mechanism of action (preclinical evidence)

NAD+ functions as an electron acceptor in catabolic redox reactions (e.g., glycolysis, citric acid cycle, β-oxidation), where it is reduced to NADH and regenerated by the electron transport chain. Beyond redox, NAD+ is a substrate for three families of regulatory enzymes: sirtuins (SIRT1-7) which deacetylate histones and metabolic targets, poly-ADP-ribose polymerases (PARPs) involved in DNA repair, and CD38/CD157 ectoenzymes that regulate cellular signaling. NAD+ is consumed by all three classes, which is why intracellular levels decline under stress and with aging.

Research applications

• Cellular bioenergetics and mitochondrial function studies
• Sirtuin enzyme activity assays (SIRT1-7)
• PARP pathway and DNA damage research
• NAD+ salvage pathway investigations
• Aging and longevity preclinical models

Storage and handling

Store lyophilised NAD+ at -20 °C in a sealed, desiccant-protected vial. NAD+ is hygroscopic and degrades to ADP-ribose under aqueous conditions, especially at neutral or alkaline pH. Reconstitute in slightly acidic buffer for best stability and use promptly.

Regulatory status

NAD+ is sold as a dietary supplement in some markets but is not an FDA-approved drug for any specific indication. Research-grade material is supplied by Peptra Labs for laboratory use only.